GSG2: Germ cell associated 2 (Haspin) kinase

2VUW: Eswaran, J., Murray, J.W., Filippakopoulos, P., Soundararajan, M., Pike, A.C.W., Von Delft, F., Picaud, S., Keates, T., King, O., Muller-Knapp, S., Lee, W.H., Marsden, B.D., Wickstroem, M., Bountra, C., Edwards, A.M., Arrowsmith, C.H., Fedorov, O., Burgess-Brown, N., Bray, J., Knapp, S.

3DLZ: Filippakopoulos, P., Eswaran, J., Keates, T., Burgess-Brown, N., Murray, J.W., Von Delft, F., Muller-Knapp, S., Lee, W.H., Marsden, B.D., Arrowsmith, C.H., Edwards, A.M., Wickstroem, M., Bountra, C., Knapp, S.

3F2N: Filippakopoulos, P., Eswaran, J., Keates, T., Burgess-Brown, N., Fedorov, O. Yue, W.W., Murray, J.W., Pike, A.C.W., Von Delft, F., Muller-Knapp, S., Lee, W.H., Marsden, B.D., Arrowsmith, C.H., Edwards, A.M., Weigelt, J., Bountra, C. Knapp, S.

3E7V: Filippakopoulos, P., Eswaran, J., Keates, T., Burgess-Brown, N., Fedorov, O., Pike, A.C.W., Von Delft, F., Muller-Knapp, S., Lee, W.H., Marsden, B.D., Arrowsmith, C.H., Edwards, A.M., Wickstroem, M., Bountra, C., Knapp, S.

PDB Codes:

2VUW (deposited on 30.May.08)

3DLZ (deposited on 30.Jun.08)

3E7V (deposited on 19.Aug.08)

3F2N (deposited on 30.Oct.08)

Datapack version: 1 (built on 24.Nov.08; last revised in 27.Feb.09)

Quick Facts

SGC target ID

GSG2A

QUICK FACTS

Haspin, Germ cell associated 2 (haspin), Haploid germ cell specific nuclear protein kinase

Updated

28. Apr. 08

Domain Structure acc. to Pfam

  • Has been shown to have Ser/Thr kinase activity

Protein Size

88.50 kDa

#residues

798 aa

Contact info.

Name: Stefan Knapp

Contact us

Entrez Gene

GSG2

cDNA available?

Synthetic

Gene sequence

NM_031965.2

Protein sequence

NP_114171

Biochemical function

  • Phosphorylation of Ser/Thr residues

Biological function

  • Regulation of diploid as well as haploid cell differentiation in a variety of tissues
  • Regulation of chromosome and spindle function during mitosis and meiosis.
  • Required for normal alignment of chromosomes at metaphase and centromeric cohesion during mitosis

Human mutations

Mouse models

Not yet available but depletion of haspin by RNAi causes misalignment of metaphase chromosomes

Summary role in disease

  • No direct disease link has been established yet but because haspin is involved in regulation of chromosomal segregation a role in cancer is likely

Closest

sequence

Matches

(10 or less)

29. Apr.08

Name

ID%

Description

NP_114171.2|

100.00

haspin [Homo sapiens]

gi|158258867|dbj|BAF85404.1|

99.87

unnamed protein product [Homo sapiens]

gi|119610887|gb|EAW90481.1|

99.75

germ cell associated 2 (haspin) [Homo sapiens]

HASP_HUMAN

99.50

Serine/threonine-protein kinase haspin (Haploid germ cell-specific nuclear protein kinase) (H-haspin) (Germ cell-specific gene 2 protein)

gi|55249571|gb|AAH47457.1|

99.37

Germ cell associated 2 (haspin) [Homo sapiens]

gi|118142841|gb|AAH16626.1|

99.22

GSG2 protein [Homo sapiens]

gi|16552166|dbj|BAB71255.1|

99.04

unnamed protein product [Homo sapiens]

XP_001159222.1|

98.62

PREDICTED: haspin [Pan troglodytes]

XP_001090778.1|

92.48

PREDICTED: similar to haspin [Macaca mulatta]

XP_854539.1|

74.65

PREDICTED: similar to haspin [Canis familiaris]

Closest match in

PDB

29. Apr.08

Name

ID%

FFAS03 Score

Description

3coi

15

-50.3

Mitogen-activated protein kinase 13

2qkr

20

-50.2

Cdc2-like CDK2/CDC28 like protein kinase

1ua2

15

-50.1

Cell division protein kinase 7

1vyw

19

-50.1

CELL DIVISION PROTEIN KINASE 2

1gii

19

-49.9

CELL DIVISION PROTEIN KINASE 2

1ogu

19

-49.8

CELL DIVISION PROTEIN KINASE 2

2r64

20

-49.8

Entity 1(prereleased)

1ob3

14

-49.7

CELL DIVISION CONTROL PROTEIN 2 HOMOLOG

1cm8

14

-49.5

PHOSPHORYLATED MAP KINASE P38-GAMMA

1jnk

14

-49.4

C-JUN N-TERMINAL KINASE

Attention.: full-length:798 residues; matches found only for the C-terminal kinase domain, ranging from ~200 to ~400 residue

Splice forms

  • Not known

ProSite hits

Accession

Description

PS50011

Protein kinase domain profile

PS00107

Protein kinases ATP-binding region signature

Protein, expression/ Localization

  • Nucleus; associated to chromosomes and found at centrosomes and spindle during mitosis

Gene expression

  • Mainly expressed in testis
  • To a lower level expressed in bone marrow, thymus adult prostate, intestine, lung, spleen and lymph node and fetal tissues

Substrates/ cofactors/ inhibitors

Interacting proteins

  • Histone H3; phosphorylates histone H3 at Thr3 during mitosis
  • c-myc

Posttranslational modifications

  • Can be phosphorylated at Thr 253
  • Autophosphorylated on both serine and threonine residues
  • Phosphorylated during mitosis
  • Phosphorylated upon DNA damage, probably by ATM or ATR

Key references

PMID: 15681610 , 11311556 , 17084365 , 15846065

Other comments

Haspin-like proteins have a complete but divergent eukaryotic protein kinase domain sequence. Haspin-related proteins lack conservation of a subset of residues that are almost invariant in known kinases and possess distinctive inserted regions. Haspin-like proteins are identified in several major eukaryotic phyla-including yeasts, plants, flies, fish, and mammals-and an extended group in Caenorhabditis elegans.

Haspin protein contains a basic region, a leucine zipper and MEFB2 homologous region in a addition to the kinase domain. Human and murine proteins are 83% identical in the C-terminal kinase domain, but only 53% identical in the N-terminal region. The Gsg2 gene is intron less and located in the intron of the alpha E2 integrin gene.